Synonymous with Rocky III, Eye of the Tiger by Survivor is a slightly harder rock piece of the 80s. Listen to it and I dare you not to start singing. Or running up a flight of stairs and jumping like a crazy person when you get to the top.
Or if you're a more recently-released Supernatural fan, it'll make you think of Dean (dammit all I couldn't find a clip of it that I could embed (see here)).